To achieve optimal results, it is essential to adjust the sonication parameters appropriately:
1. Amplitude: Higher amplitudes generate stronger shear forces but can also lead to greater heat production. 2. Time: The duration of sonication should be optimized to balance between effective dispersion and avoiding excessive heat buildup. 3. Pulse Mode: Using pulse mode (alternating between on and off cycles) can help manage heat generation and improve dispersion efficiency. 4. Temperature Control: Employing cooling systems or performing sonication in an ice bath can mitigate heat-related issues.
