The basic procedure involves culturing cells in the presence of the nanomaterial under investigation. After a certain period, the cells are treated with a cytochalasin-B to inhibit cytokinesis, which allows for the identification of binucleated cells. These cells are then stained and examined under a microscope to count the number of micronuclei. The frequency of micronuclei is compared to control samples to determine the genotoxic potential of the nanomaterial.
