The field of advanced imaging is continuously evolving, with several recent advancements: - Cryo-Electron Microscopy (Cryo-EM): This technique allows for the imaging of biological macromolecules in their native state at near-atomic resolution. - Super-Resolution Microscopy: Techniques like STED, PALM, and STORM have broken the diffraction limit of light, enabling imaging at resolutions previously thought impossible. - In-situ and Operando Techniques: These methods allow for the real-time observation of nanomaterials under working conditions, providing insights into their dynamic behaviors.