Template Selection: Identify a template protein with a known structure that is homologous to the target protein. Sequence Alignment: Align the sequence of the target protein with the sequence of the template protein. Model Building: Generate a 3D model of the target protein based on the alignment with the template structure. Model Refinement: Optimize the model to improve its accuracy, often using energy minimization techniques. Model Validation: Assess the quality of the model using various validation tools and criteria.
