In the context of nanotechnology, HCS typically involves: 1. Preparation of Nanoparticles: Synthesis and characterization of nanoparticles with desired properties. 2. Cell Culturing: Cultivation of cells that will be exposed to nanoparticles. 3. Exposure and Treatment: Introducing nanoparticles to cell cultures under controlled conditions. 4. Imaging: Using high-resolution microscopy to capture images of cells post-treatment. 5. Image Analysis: Employing image analysis software to quantify cellular responses such as changes in morphology, viability, and intracellular signaling.
