Nanoparticles can interfere with assays in several ways:
Optical Interference: Nanoparticles can absorb or scatter light, affecting spectrophotometric and fluorescent measurements. This is particularly problematic in assays that rely on optical detection. Chemical Interference: The surface chemistry of nanoparticles can interact with assay components, leading to non-specific binding or altering the chemical environment. Physical Interference: The physical presence of nanoparticles can influence the spatial distribution of molecules, affecting the assay's dynamics and results.
