There are several strategies to minimize autofluorescence in nanotechnological applications:
Spectral Unmixing: Using advanced imaging techniques to separate the autofluorescence signal from the signal of interest. Chemical Treatment: Applying quenching agents to reduce autofluorescence. Optical Filters: Employing filters to block specific wavelengths of light that contribute to autofluorescence. Time-Gated Detection: Leveraging the difference in the lifetimes of autofluorescent molecules and fluorescent tags to discriminate between them.
