Design and Synthesis: The initial phase focuses on designing the nanocarrier structure and selecting appropriate materials. Techniques like
self-assembly and chemical synthesis are commonly used.
Drug Loading: The therapeutic agent is incorporated into the nanocarrier through various methods, such as encapsulation, adsorption, or covalent attachment.
Characterization: Advanced analytical techniques, including
dynamic light scattering and electron microscopy, are employed to assess the size, shape, and surface properties of the nanocarriers.
In Vitro and In Vivo Testing: Nanocarriers undergo rigorous biological testing to evaluate their stability, toxicity, and efficacy. This includes cell culture studies and animal models.
Optimization and Scale-Up: Based on the test results, the nanocarrier formulation is optimized for improved performance and scaled up for potential clinical use.
