The synthesis of liposomes involves several methods, including thin-film hydration, reverse-phase evaporation, and solvent injection techniques. The choice of method depends on the desired size, lamellarity (number of bilayers), and encapsulation efficiency. _Thin-film hydration_ is one of the most common methods, where a lipid film is hydrated with an aqueous solution to form multilamellar vesicles, which can then be processed into smaller unilamellar vesicles.
