The measurement of inflammatory markers can be achieved through various techniques, including:
1. Enzyme-Linked Immunosorbent Assay (ELISA): A popular method for quantifying specific cytokines in cell culture supernatants, blood, or other biological fluids. 2. Flow Cytometry: Used to analyze the expression of inflammatory markers on the surface of immune cells and intracellular cytokine production. 3. Multiplex Assays: These assays allow the simultaneous measurement of multiple cytokines and chemokines from a single sample, providing a comprehensive profile of the inflammatory response. 4. Real-Time PCR: This technique measures the mRNA levels of inflammatory markers, providing insights into the transcriptional regulation of the inflammatory response.
