How are Cells Prepared for Nanotechnology Applications?
The process of cell preparation involves several steps:
Cell Isolation Cells can be isolated from various tissues or obtained from cell lines. The isolation method depends on the cell type and the intended application. For example, magnetic-activated cell sorting (MACS) or fluorescence-activated cell sorting (FACS) can be used to isolate specific cell populations.
Cell Culture Once isolated, cells are cultured in a controlled environment. This involves providing the cells with the necessary nutrients, growth factors, and conditions such as temperature and CO2 levels. The culture medium and conditions may vary depending on the cell type.
Cell Maintenance Maintaining cell cultures involves regular monitoring and changing of the culture medium to ensure optimal growth conditions. It also includes subculturing or passaging cells to prevent over-confluence and maintain healthy cell populations.
