What Techniques are Used in Single Molecule Studies?
Several sophisticated techniques are employed to study single molecules:
Atomic Force Microscopy (AFM): Utilizes a cantilever to interact with molecules and provides topographical and mechanical information. Fluorescence Microscopy: Allows for the visualization of individual molecules using fluorescent tags. Optical Tweezers: Use laser beams to manipulate single molecules with high precision. Scanning Tunneling Microscopy (STM): Provides atomic resolution images by measuring the tunneling current between a sharp tip and the molecule. Single-Molecule FRET: Measures energy transfer between two fluorophores to study molecular interactions and dynamics.
