Several advanced techniques are employed in nanobiophysics, including:
Atomic Force Microscopy (AFM): Allows for the imaging and manipulation of individual molecules and complexes. Cryo-Electron Microscopy (Cryo-EM): Provides high-resolution images of biological specimens in their native state. Single-Molecule Fluorescence: Enables the observation of individual molecules in real-time, providing insights into their behavior and interactions. Nanopore Sequencing: A technique for sequencing DNA by passing it through a nanoscale pore, offering a fast and accurate method of genomic analysis. Nanoscale Spectroscopy: Techniques such as Raman spectroscopy can provide detailed information about molecular composition and dynamics at the nanoscale.