Several methods are employed for particle counting, each with its own advantages and limitations:
1. Dynamic Light Scattering (DLS): This technique measures the scattering of light by particles in suspension to determine their size distribution. 2. Nanoparticle Tracking Analysis (NTA): NTA visualizes and tracks the Brownian motion of nanoparticles to measure their size and concentration. 3. Electron Microscopy: Techniques like Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) provide detailed images of nanoparticles, allowing for precise size measurement. 4. Coulter Counter: This method uses electrical impedance to count and size particles in a fluid. 5. Flow Cytometry: Commonly used in biological applications, this technique analyzes the physical and chemical characteristics of particles in a fluid as they pass through a laser.