transmission electron microscope (tem)

What are the Limitations of TEM?

Despite its capabilities, TEM has some limitations:
- Sample Preparation: Samples must be extremely thin (less than 100 nanometers) and carefully prepared, which can be time-consuming and challenging.
- Vacuum Requirement: TEM operates in a high vacuum environment, making it unsuitable for observing samples in their natural state.
- Radiation Damage: The high-energy electron beam can damage sensitive samples, limiting the observation time and detail.

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