What are the Limitations of Laser Scanning Microscopy?
While laser scanning microscopy is a powerful tool, it does have some limitations:
Depth Penetration: The depth of penetration in thick samples can be limited, affecting the ability to image internal structures. Fluorescence Bleaching: Prolonged exposure to laser light can cause fluorescence bleaching, affecting image quality. Cost: High-quality laser scanning microscopes can be expensive, which might be a barrier for some research labs.
