Despite its advantages, HEED has some limitations:
1. Sample Preparation: High-quality sample preparation is crucial for obtaining accurate results. Samples must be thin enough to allow electron transmission, which can be challenging for some materials. 2. Complex Data Interpretation: The diffraction patterns can be complex and require sophisticated analysis techniques to interpret accurately. 3. Instrumentation Costs: HEED requires specialized equipment, which can be expensive and may not be readily available in all research settings.