Characterization of the nanomaterial's physical and chemical properties. Assessment of cytotoxicity, or the potential of the nanomaterial to cause cell damage. Evaluation of potential genotoxicity, which refers to the ability of the nanomaterial to cause genetic mutations. Testing for immunogenicity, or the likelihood that the nanomaterial will trigger an immune response. Evaluation of degradation and biopersistence, determining how the nanomaterial breaks down and how long it remains in the body.