What are the Challenges in Single Molecule Imaging?
Despite its powerful capabilities, single molecule imaging faces several challenges:
Photobleaching: Fluorophores can lose their ability to fluoresce over time, limiting long-term observations. Signal-to-noise ratio: Achieving a high signal-to-noise ratio is critical for accurate detection but can be difficult in complex biological environments. Temporal resolution: Balancing temporal resolution with spatial resolution to capture fast molecular dynamics.
