What are the Challenges in Optical Imaging at the Nanoscale?
Despite its advantages, optical imaging at the nanoscale faces several challenges, including:
- Diffraction Limit: Conventional optical microscopy is limited by the diffraction of light, restricting resolution to about 200 nm. - Photobleaching: Fluorescent dyes can degrade over time, reducing the quality of imaging. - Sample Preparation: Preparing samples without altering their properties can be challenging. - Signal-to-Noise Ratio: Achieving a high signal-to-noise ratio for clear imaging can be difficult.
