1. Template Leakage: Residual template molecules may remain in the polymer matrix, leading to false positives in sensing applications. 2. Complex Template Molecules: Synthesizing MIPs for large or complex molecules can be challenging due to difficulties in creating accurate binding sites. 3. Reusability: While MIPs are stable, the reusability of these polymers can be limited by the robustness of the binding sites after multiple cycles.
