What Are the Challenges in Fluorescence Thermometry?
Despite its advantages, fluorescence thermometry faces several challenges:
Photobleaching: The fluorescent materials can degrade over time due to prolonged exposure to light, affecting accuracy. Calibration: Accurate temperature measurement requires precise calibration of the fluorescence response to temperature changes. Environmental Sensitivity: Factors such as pH, ionic strength, and local environment can influence fluorescence, complicating measurements.
