Cell Seeding: Cells are seeded into a 96-well plate and allowed to adhere and grow. Treatment: Cells are treated with nanoparticles or other test substances. MTT Addition: The MTT reagent is added, and cells are incubated to allow for metabolization. Solubilization: Formazan crystals formed by viable cells are dissolved using a solubilizing agent (e.g., DMSO). Measurement: The absorbance is measured using a spectrophotometer, which correlates with the number of viable cells.
