LOD is typically determined via a combination of experimental methods and statistical analysis. Here are some general steps:
1. Calibration Curve: A calibration curve is created by measuring the response of the sensor to known concentrations of the analyte. 2. Blank Measurements: Measurements are taken with no analyte present to determine the baseline noise. 3. Statistical Analysis: The LOD is often calculated as the concentration at which the signal is three times the standard deviation of the blank measurements (3σ rule).
