TOF analyzers operate on the principle that ions with different masses travel at different speeds when subjected to the same kinetic energy. Here’s a step-by-step breakdown:
Ionization: The sample is ionized using techniques like Matrix-Assisted Laser Desorption/Ionization (MALDI) or Electrospray Ionization (ESI). Acceleration: The ions are accelerated by an electric field, imparting them with the same kinetic energy. Flight: The ions travel through a flight tube. Lighter ions travel faster and reach the detector sooner than heavier ions. Detection: The time it takes for each ion to reach the detector is recorded, allowing the mass-to-charge ratio to be determined.
