How Does Super-Resolution Microscopy Break the Diffraction Limit?
Super-resolution microscopy techniques overcome the diffraction limit of light, which restricts conventional optical microscopy to a resolution of about 200 nanometers. Techniques like STED use targeted light emission to deplete fluorescence in specific areas, effectively sharpening the image. PALM uses the precise activation and localization of individual fluorescent molecules to construct high-resolution images. These methods enable the study of complex biological structures and dynamic processes at the nanoscale.
