In Real-Time PCR, the process begins with the extraction of DNA from a sample. This DNA is then mixed with specific primers, nucleotides, and a DNA polymerase enzyme. During the thermal cycling process, the DNA is denatured, annealed, and extended, leading to the amplification of the target DNA sequence. The real-time aspect comes from the use of fluorescent dyes or probes that emit a signal proportional to the amount of DNA being amplified, allowing for the continuous monitoring of the reaction.
