Microfluidic chromatography involves the use of microchannels etched into a substrate, typically made of materials like polydimethylsiloxane (PDMS), glass, or silicon. These channels are designed to facilitate the flow of fluids and the separation of compounds based on their interactions with the channel surfaces and the mobile phase. The process typically includes:
1. Sample Injection: A small volume of the sample is introduced into the microfluidic chip. 2. Separation: The sample is carried through the microchannels by a mobile phase (liquid or gas), where different components are separated based on their physical and chemical properties. 3. Detection: Separated components are detected using various methods such as optical detection, mass spectrometry, or electrochemical detection.
