cryo electron tomography (cryo et)

How Does Cryo Electron Tomography Work?

Cryo ET involves several key steps:
1. Specimen Preparation: Samples are rapidly frozen to cryogenic temperatures to prevent ice crystal formation, preserving the native state.
2. Data Collection: An electron beam is transmitted through the frozen sample, and multiple images are captured at different tilt angles.
3. Image Reconstruction: Specialized software combines these 2D images to generate a 3D reconstruction of the sample.

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