The working mechanism of a Nano Pen involves the use of a sharp tip, often made from materials like Silicon or Carbon Nanotubes, which interacts with the substrate at a nanometer scale. The tip can be controlled with high precision through Piezoelectric Actuators, allowing for the deposition of various materials such as Metals, Polymers, and Biomolecules. This precise control enables the creation of intricate nanoscale patterns and structures.