The process of using cloning vectors involves several steps:
1. Isolation of Vector and Insert DNA: The vector DNA and the DNA to be cloned (insert DNA) are isolated. 2. Digestion with Restriction Enzymes: Both vector and insert DNA are cut with the same restriction enzymes to create compatible ends. 3. Ligation: The insert is then ligated into the vector using DNA ligase. 4. Transformation: The recombinant DNA is introduced into a host cell through a process called transformation. 5. Selection: Host cells that have successfully taken up the recombinant DNA are selected using marker genes.
