Anti-fade reagents typically work by scavenging reactive oxygen species (ROS), which are the primary culprits behind photobleaching. Some anti-fade reagents function by providing a protective layer around the fluorescent molecules, while others act as antioxidants to neutralize ROS. Common anti-fade reagents include p-phenylenediamine (PPD), ascorbic acid (Vitamin C), and glutathione (GSH).
