Several strategies can be employed to minimize the impact of background autofluorescence:
Choice of fluorophores: Selecting fluorophores that emit light at wavelengths different from those of the autofluorescent components can help reduce interference. Spectral unmixing: Advanced imaging techniques can separate the autofluorescent signal from the specific signal of interest based on their distinct spectral properties. Sample preparation techniques: Using alternative fixatives and embedding media that exhibit low autofluorescence can also help. Optical filters: Employing filters that selectively transmit the emission wavelengths of the fluorophores while blocking those of autofluorescent components can improve the signal-to-noise ratio.
