Nanoliposomes are typically formed through methods such as thin-film hydration, solvent injection, and reverse-phase evaporation. In thin-film hydration, lipids are dissolved in an organic solvent, which is then evaporated to form a thin film. This film is hydrated with an aqueous solution, resulting in the formation of nanoliposomes. The size and characteristics of the nanoliposomes can be controlled by techniques like sonication and extrusion.
