The process typically involves the functionalization of nanoparticles with specific chemical groups to enable the attachment of antibodies. Common methods include: - Covalent Bonding: Using linkers like EDC/NHS to create stable bonds between the nanoparticle and the antibody. - Adsorption: Relying on physical interactions to attach antibodies to the nanoparticle surface. - Biotin-Streptavidin Binding: A highly specific interaction used to link biotinylated antibodies with streptavidin-coated nanoparticles.
