Silver Nanoparticles in Cancer Therapy: Exploring Biocompatibility and Cytotoxicity

Introduction

Cancer is still considered one of the major threats to human health and life, and the need for new, efficient treatments never ceases. Conventional external beam radiation in combination with chemotherapy has advanced patient prognosis care but exhibits drastic adverse effects and poor target selectivity. Here enters nanotechnology, a new and fascinating area in medicine. This paper focuses on the synthesis of silver nanoparticles (AgNPs) as a potential agent in cancer therapy because of their minimal toxicity, specificity in cancer cell killing, and eco-friendly synthesis techniques. This article focuses on the aspect of cancer therapy and here takes up a detailed study of the distinctive ability of silver nanoparticles and their biocompatibility and cytotoxicity in different contexts to contribute to the set development of cancer therapy.

The Promise of Silver Nanoparticles in Cancer Treatment

The antimicrobial properties of silver nanoparticles are well explored, but the uses of nanoparticles are not limited to infection control. In cancer therapy, there is a great resource in the inherent characteristics of the AgNPs, including size, shape, and surface charge. These nanoparticles can be designed to attack cancerous tissues with little interaction with healthy ones, hence reducing the harm to the healthy tissues. Also, they can be obtained through green processes, which include plant origin, microorganisms, and fungi, making these nanomaterials more attractive for medicinal use.

Thus, one of the major remarkable properties of AgNPs is the capacity to provoke apoptosis of cancer cells through ROS production and DNA fragmentation. This mode of action makes it possible to achieve a more selective action on tumor cells, excluding systemic toxicity—side effects typical of traditional treatments. Moreover, since it almost has no side effects on healthy cells, the nanocarriers are very effective in cancer therapies.

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Mechanisms of Cytotoxicity: How Silver Nanoparticles Target Cancer Cells

ROS Generation

It has been found that silver nanoparticles can cause oxidative stress in cancer cells and thereby form ROS. This oxidative stress interferes with the normal cellular redox balance and also bleaches cell proteins, lipids, and DNA. In cancer cells, this damage results, for example, in the triggering of programmed cell death called apoptosis, which in turn stops tumor growth.

DNA Interaction and Damage

It has been found that AgNPs can bind to the cellular DNA directly. These interactions can disrupt DNA’s double helix structure and can also prevent the replication of cancer cells. Moreover, the present nanoparticles can selectively locate and attach to cancerous DNA, a feature that makes them suitable for targeted therapies.

Disruption of Mitochondrial Function

Among the intracellular targets of silver nanoparticles, mitochondria deserve special attention since they are the energy centers of the cell. The present study demonstrated that AgNPs disrupt mitochondrial function and contribute to energy failure and cell death. This disruption is most effective in cancer cells since most of them densely depend on mitochondrial metabolism.

Induction of Apoptosis

Silver nanoparticles induce apoptosis through intrinsic as well as extrinsic mechanisms. AgNPs throw disruptions in the cell signaling networks and generate caspases that allow the death of tumor cells but not the healthy ones.

Biocompatibility of Silver Nanoparticles: Ensuring Safe Application

The use of any therapeutic agent depends on the toxicity of the same. Biocompatibility of the nanoparticles is, therefore, an essential factor in evaluating the practicability of these silver nanoparticles in hospital environments. Research has shown that AgNPs prepared by green approaches—that is, the use of plant extracts and biomolecules—are highly bio-friendly. These nanoparticles do not readily provoke adverse immune reactions and can, therefore, be used in therapeutic approaches.

Also, the size, the charge of the surface, and the coating of the silver nanoparticles affect its response to biological entities. For example, small particles and highly charged particle surfaces are less toxic to nontumorous cells but effective against cancer cells. Such a bimodal nature highlights the criticality of having an optimum nanoparticle to serve the best therapeutic effects with the fewest side effects.

Advances in Silver Nanoparticle Synthesis for Cancer Therapy

Green Synthesis

Green synthesis using extracts of plants, fungi, and bacteria to synthesize silver nanoparticles has also been on the rise. These methods are non-hazardous to the environment and reduce the use of hazardous chemical reagents. Further, the biological molecules in these extracts help in reducing and stabilizing the nanoparticles, providing special features to nanoparticles, and improving the therapeutic properties.

Shape and Size Optimization

The size and morphology of the synthesized AgNPs have contributed significant factors that influence cytotoxicity. The samples, such as spherical and small-sized nanoparticles, have a greater cellular uptake and a higher degree of cytotoxicity. This optimization makes sure that the nanoparticles get to the tumor’s cells to the maximum extent possible.

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Applications in Cancer Therapy: Case Studies and Insights

Breast Cancer

For example, fifty nm silver nanoparticles have also revealed the pronounced cytotoxic influence over the breast cancer cell line, viz., MDA-MB-231. Thus, through the process of apoptosis and cell growth inhibition, AgNPs appear to be an acceptable complementary or potential substitute therapy.

Liver and Lung Cancers

Cytotoxicity of AgNPs has been performed against liver (Hep G2) and lung (L-132) cancer cell lines. These nanoparticles are very selective towards cancer cells and post low harm to the rest of the cells in the body.

Cervical and Prostate Cancers

Researchers established that incorporated AgNPs have cytotoxic effects on cervical (HeLa) and prostate (PC-3) cancer cell lines. That is why they are such powerful anticancer agents, regulating oxidative stress and interfering with standard cell processes.

Pancreatic and Oral Cancers

The potentiality of silver nanoparticles also exists for the repression of difficult-to-treat cancers such as pancreatic (MIA-Pa-Ca-2) and oral (KB) cells. This is because AgNPs directly affect cancer cells, only without harming healthy cells—a factor that sets them apart from other chemotherapeutic solutions.

Challenges and Future Directions

Although it seems that silver nanoparticles are highly attractive for use in clinical work, their application has some drawbacks. The toxicity of AgNP and its effects on tissues and organisms, in the long run, deserves to be studied stringently. Second, there is still a problem of how to produce nanoparticles en masse and make them a standard product that can be used by many manufacturers.

Future research should focus on:

Designing and synthesizing novel polyfunctional nanoparticles having incorporated diagnostic and therapeutic functionalities.

Exploring how AgNPs interact with other cancer treatments and what are the outcomes.

Improving the targeting procedures so that only malignant cells are affected.

Conclusion

Silver nanoparticles bring a revolutionary change in cancer treatment. These features, from localized cell killing to non-toxicity, have made them a potent weapon against cancer as categorized. While researchers go on exploring their full potential, AgNPs will revolutionize cancer treatment, opening a door to more efficient and noninvasive methods.

References

  1. Jadhav, K., Deore, S., Dhamecha, D., Hr, R., Jagwani, S., Jalalpure, S. and Bohara, R., 2018. Phytosynthesis of silver nanoparticles: characterization, biocompatibility studies, and anticancer activityACS Biomaterials Science & Engineering4(3), pp.892-899.
  2. He, Y., Wei, F., Ma, Z., Zhang, H., Yang, Q., Yao, B., Huang, Z., Li, J., Zeng, C. and Zhang, Q., 2017. Green synthesis of silver nanoparticles using seed extract of Alpinia katsumadai, and their antioxidant, cytotoxicity, and antibacterial activities. RSC advances7(63), pp.39842-39851.
  3. Verma, S.K., Jha, E., Sahoo, B., Panda, P.K., Thirumurugan, A., Parashar, S.K.S. and Suar, M., 2017. Mechanistic insight into the rapid one-step facile biofabrication of antibacterial silver nanoparticles from bacterial release and their biogenicity and concentration-dependent in vitro cytotoxicity to colon cells. RSC advances7(64), pp.40034-40045.
  4. Rai, M.K., Deshmukh, S.D., Ingle, A.P. and Gade, A.K., 2012. Silver nanoparticles: the powerful nanoweapon against multidrug‐resistant bacteria. Journal of applied microbiology112(5), pp.841-852.
  5. Rahban, M., Divsalar, A., Saboury, A.A. and Golestani, A., 2010. Nanotoxicity and spectroscopy studies of silver nanoparticle: calf thymus DNA and K562 as targets. The Journal of Physical Chemistry C114(13), pp.5798-5803.
  6. Ivask, A., Kurvet, I., Kasemets, K., Blinova, I., Aruoja, V., Suppi, S., Vija, H., Käkinen, A., Titma, T., Heinlaan, M. and Visnapuu, M., 2014. Size-dependent toxicity of silver nanoparticles to bacteria, yeast, algae, crustaceans and mammalian cells in vitro. PloS one9(7), p.e102108.
  7. Gomaa, E.Z., 2017. Silver nanoparticles as an antimicrobial agent: A case study on Staphylococcus aureus and Escherichia coli as models for Gram-positive and Gram-negative bacteria. The Journal of general and applied microbiology63(1), pp.36-43.
  8. Kelkawi, A.H.A., Abbasi Kajani, A. and Bordbar, A.K., 2017. Green synthesis of silver nanoparticles using Mentha pulegium and investigation of their antibacterial, antifungal and anticancer activity. IET nanobiotechnology11(4), pp.370-376.
  9. Lopes, L.C., Brito, L.M., Bezerra, T.T., Gomes, K.N., Carvalho, F.A.D.A., Chaves, M.H. and Cantanhede, W., 2018. Silver and gold nanoparticles from tannic acid: synthesis, characterization and evaluation of antileishmanial and cytotoxic activities. Anais da Academia Brasileira de Ciências90(03), pp.2679-2689.

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